Effect of Liver Ischemia-Reperfusion Injury on Genotoxicity in Rabbits: Experimental Study

Objective: Ischemia-reperfusion (I-R) injury is defined as the paradoxical exacerbation of cellular dysfunction and death following restoration blood flow to ischemic tissues. In our study, it was aimed examine potential DNA effects liver IR with an experimental animal model. Material Methods: modeling done seven male New Zealand rabbits. Blood samples were taken before model, 30 minutes after ischemia, 60 reperfusion. The damage in rabbits measured using Tail Length, Intensity, Moment techniques. Statistical significance determined one-way analysis variance (ANOVA) Tukey's post hoc test. Results: There are significant differences between control-ischemia, control-reperfusion I-R groups all 3 measurements. length; increased by 51.84%, 54.16% ischemia reperfusion, respectively. length 134.09% control Similarly, tail density moment 78.95% (after ischemia), 77.96% reperfusion), 85.54% 165.52% reperfusion) Conclusion: Tissue disruption known occur tissue hypoxia that triggers anaerobic respiration. Restoring a hypoxic-tissue results increase reactive oxygen species production. Literature stated I/R-related may result from formation radicals during reperfusion period. Moreover, induces oxidative exceeds antioxidative capacity circulating leukocytes, leading damage. lesions characteristic mediated free detected at significantly level